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More than 86% of treated participants lost at least 5% of body weight

A moderate deficit of 500750 calories per day is generally considered appropriate for sustainable fat loss without excessive lean mass sacrifice

they're signaling molecules

Nevertheless, when analyses were adjusted for BMI, level of physical activity, number of somatic comorbidities, smoking status and alcohol use, results were substantially unchanged

This manifests as deeper G/G arrest, more stringent S phase depletion, and increased late apoptosis, representing redox-coupled modulation of genotoxic stress rather than simple damage amplification

Clinical assessments: A complete physical examination was performed before enrollment